Sino Biological, Inc. (Shenzhen Stock Exchange: 301047.SZ) announced that its gene synthesis and cell-free protein expression workflow was used in a recent study by Tencent AI for Life Sciences Lab, published in Nature Communications. The study demonstrated rapid experimental validation of AI-designed proteins with improved activity, stability, and multifunctionality, addressing a key challenge in protein engineering.
Artificial intelligence has significantly accelerated protein amino acid sequence design, but translating computational designs into functional proteins remains difficult. Protein activity, stability, folding, and expression are influenced by complex structural and biochemical factors, often leading to discrepancies between in silico predictions and experimental outcomes. To bridge this gap, the study introduced an Ontology Reinforcement Iteration (ORI) framework, integrating protein ontology with reinforcement learning from wet-lab feedback. Experimental data, including protein expression levels and functional activity, were continuously fed back into the model, enabling iterative optimization of protein sequences and improved design accuracy.
The researchers utilized Sino Biological’s XPressMAX™ Cell-Free Protein Synthesis Kit to enable rapid protein expression and functional screening. Protein-coding sequences were cloned into the kit’s expression vector and added to the proprietary cell-free reaction system, supporting rapid design–build–test cycles. Using this workflow, the team engineered a lysozyme with over 100-fold higher activity than the natural enzyme, developed a thermostable chitinase retaining activity at 85°C, and successfully expressed bifunctional enzymes with improved performance compared with naturally occurring multifunctional enzymes.
The XPressMAX™ Cell-Free Protein Synthesis Kit offers ultra-fast synthesis, with protein expression completed in as little as 3 hours. It provides high screening efficiency, optimized for VHH, scFv, and miniproteins, and is compatible with plasmid and linear DNA templates. The kit is disulfide bond friendly, enabling expression of complex disulfide-bonded proteins without additional enhancers, and offers cost-effective performance with reduced operational time and cost. It is suitable for high-throughput and industrial applications with scalable supply.
Sino Biological is an international reagent supplier and CRO service provider specializing in recombinant protein production and antibody development. With the US-based Center for Bioprocessing (C4B) in Houston and SignalChem Biotech (part of Sino Biological) in Canada, the company delivers tailored, localized solutions to meet diverse research needs worldwide. Serving researchers in over 90 countries, Sino Biological maintains a stringent quality management system across all products. The study highlights the potential of integrating AI design with high-throughput experimental validation to accelerate protein engineering.
